Capto core 700 resin1/13/2024 Small contaminant molecules enter into the beads where they are captured. The Capto Core 700 medium has a core bead design and consists of an inactive shell and a ligand-activated core. Three alternative process streams for the purification of influenza A/H1N1 were evaluated in this study. In GF, the low productivity relates to low flow rates and limited sample loads. In these processes, both sucrose density gradient ultracentrifugation and GF have limitations in, for example, scalability and productivity. The methods for purification processes have typically involved a combination of sucrose density gradient ultracentrifugation, ultrafiltration/diafiltration (UF/DF) with hollow-fiber membranes, and chromatography using affinity-, ion exchange-, and/or gel filtration (GF) media. However, to meet the needs for pandemic preparedness and scalability of vaccine productions, cell-based processes are being developed and implemented to a greater extent in the industry. Introduction Influenza vaccine has historically and is today primarily produced in embryonated chicken eggs. This study shows that while offering the same purity as gel filtration, Capto Core 700 enables significant improvements in productivity and process economy. Here, the performance of Capto Core 700 in three different processes for the purification of influenza H1N1 virus from infected mammalian cells was evaluated and compared. The size separating mode of operation could be compared to gel filtration (size exclusion chromatography), which is a common approach for polishing steps in vaccine processes. Capto Core 700 is built on the core bead technology which allows for dual functionality combining size separation with binding chromatography. The medium is designed to be used in flowthrough mode for large targets (> M r 700 000) while scavenging smaller contaminants. GE Healthcare Life Sciences Application note 29-0003-34 AA Vaccines Purification of influenza A/H1N1 using Capto Core 700 Capto Core 700 is a chromatography medium (resin) optimized for purification of viruses and other large biomolecules.
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